The Lawrence Jacobsen Library
Books Received (Primate-Science/PrimateLit)

KEY EXPERIMENTS IN PRACTICAL DEVELOPMENTAL BIOLOGY

 Edited by Manuel Mari-Beffa and Jennifer Knight 
 Cambridge University Press

ABOUT THE BOOK
 This unique resource presents 27 easy-to-follow laboratory exercises for use
 in student practical classes, all of which are classic experiments in developmental
 biology. These are experiments that provided key insights into developmental
 questions, and many of them are described by the leaders in the field who carried
 out the original pioneering research. This book intends to bridge the gap between
 state-of-the-art experimental work and the laboratory classes taken at the undergraduate
 and post-graduate levels. All chapters follow the same logical format, taking
 the students from materials and methods, through results and discussion, so
 that they learn the underlying rationale and analysis employed in the research.
 The book will be an invaluable resource for graduate students and instructors
 teaching practical developmental biology courses. Chapters also include teaching
 concepts, discussion of the degree of difficulty of each experiment, potential
 sources of failure, as well as the time required for each experiment to be carried
 out in a practical class with students.

 ABOUT THE EDITORS
 Manuel Mari-Beffa is a Lecturer in Developmental Biology at the University of
 Malaga. 
 
Jennifer Knight is an Instructor in the Department of Molecular, Cellular and 
Developmental Biology at the University of Colorado, Boulder. 
 

TABLE OF CONTENTS
 
List of contributors xi 
 
Preface xv 
  
MANUEL MARI-BEFFA 
 
Introduction 1 
 
JENNIFER KNIGHT 
  
SECTION 1. GRAFTINGS 
  
1. Two developmental gradients control head reformation in hydra 4 
 
H. R. BODE 
 
2. Embryonic regulation and induction in sea urchin development 23 
  
C. A. ETTENSOHN 
  
3. The isthmic organizer and brain regionalization in chick embryos 37 
  
D. ECHEVARRiA and S. MARTINEZ 
  
SECTION 11. SPECIFIC CHEMICAL REAGENTS 
 
4. Chemotaxis of aggregating Dictyostelium cells 50 
  
G. GERISCH and M. ECKE 
  
5. Inhibition of signal transduction pathways prevents head  regeneration in hydra 67 
  
L. M. SALGADO 
  
6. Retinoic acid during limb regeneration 77 
 
M. MADEN 
  
SECTION III BEAD IMPLANTATION 
  
7. Experimental manipulations during limb development in avian embryos 85 
 
Y. GANAN, J. RODRIGUEZ-LEON, and D. MACIAS 
  
8. Induction of ectopic limb outgrowth in chick with FGF-8 
  
A. RAYA, C. RODRIGUEZ ESTEBAN, and J. C. ~ZPISUA-BELMONTE 99 
 
SECTION IV. NUCLEIC ACID INJECTIONS 
 
9. RNAi techniques applied to freshwater planarians (Platyhelminthes) during regeneration 106 
 
D. BUENO, R. ROMERO, and E. SALO 
 
10. Microinjection of Xenopus embryos 117 
 
R. J. GARRIOCK and P. A. KRIEG 
  
SECTION V. GENETIC ANALYSIS 
 
11. Segmental specification in Drosophila melanogaster 127 
  
L. DE NAVAS, M. SUZANNE, D. FORONDA, and E. SANCHEZ-HERRERO 
  
12. Genetic analysis of flower development in Arabidopsis thaliana. 143 
  
The ABC model of floral organ identity determination 
  
J. L. RIECHMANN 
  
13. Genetic analysis of vulva development in C. elegans 153 
 
S. CANEVASCINI 
 
SECTION VI. CLONAL ANALYSIS 
 
14. The role of the gene apterous in the development of the Drosophila wing 167 
 
E J. DiAZ-BENJUMEA 
 
15. Extramacrochaetue, an example of a gene required for control of limb 178 
 
size and cell differentiation during wing morphogenesis in Drosophila 
 
BAONZA 
 
16. Hedgehog transduction pathway is involved in pattern formation 190 
 
of Drosophila melanogaster tergites 
 
M. MARI-BEFFA 
 
SECTION Vll. IN SITU HYBRIDIZATION 
 
17 Retinoic acid signalling controls anteroposterior patterning of the 205 
 
zebrafish hindbrain 
 
G. BEGEMANN 
 
18. Left-right asymmetry in the mouse 217 
M. BLUM, A. SCHWEICKERT, and C. KARCHER 
 
SECTION VIII. TRANSGENIC ORGANISMS 
 
19. Bicoid and Dorsal: Two transcription factor gradients that specify cell 231 
 
fates in the early Drosophila embryo 
 
S. ROTH 
 
20. Significance of the temporal modulation of Hox gene expression on 255 
 
segment morphology J. CASTELLI-GAIR HOMBRIA 
 
21. The UAS/GAL4 system for tissue-specific analysis of EGFR gene   269 
 
function in Drosophila melanogaster 
 
J. B. DUFFY and N. PERRIMON 
 
22. Neurogenesis in Drosophila: A genetic approach 282 
 
C. KLAMBT and H. VAESSIN 
 
23. Role of the achaete-scute complex genes in the development of 296 
 
the adult peripheral nervous system of Drosophila melanogaster 
 
S. SOTILLOS and S. CAMPUZANO 
 
SECTION IX. VERTEBRATE CLONING 
 
24. The conservation of the genome and nuclear reprogramming in 310 
 
Xenopus 
 
J. B. GURDON 
 
SECTION X. CELL CULTURE 
 
25. In vitro culture and differentiation of mouse embryonic stem cells 316 
 
A. ROLLETSCHEK, C. WIESE, and A. M. WOBUS 
 
SECTION XI. EVO-DEVO STUDIES 
 
26. Microevolution between Drosophila species 330 
 
N. SKAER and P. SIMPSON 
 
SECTION Xll. COMPUTATIONAL MODELLING 
 
27. Theories as a tool for understanding the complex network of 346 
 
molecular interactions 
 
H. MEINHARDT 
 
Appendix 1. Abbreviations 355 
 
Appendix 2. Suppliers 363 
 
Index 371 
 
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HOW TO ORDER 
 
ISBN 0-521-83315-9 
 
$100.00, Hardcover 
 
Cambridge University Press 
40 West 20th Street 
New York, NY 10011-4211 
  
(212) 227-5057 
 
http://www.cambridge.org/uk/catalogue/catalogue.asp?isbn=0521833159